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E-GEOD-32265 - Gene-expression changes resulting from loss of the mTORC1 component Raptor in murine hematopoietic stem and progenitor cell-enriched populations (HSPC)

Status
Released on 19 September 2012, last updated on 24 September 2012
Organism
Mus musculus
Samples (6)
Array (1)
Protocols (8)
Description
We investigated the role of mTORC1 in murine hematopoiesis by conditionally deleting the Raptor gene in murine hematopoietic stem cells. We observed mutliple alterations evoked by Raptor loss in hematopoiesis and profiled gene-expression alterations induced by raptor loss in Flt3-Lin-Sca1+cKit+ hematopoietic stem and progenitor enriched cell populations, 5 weeks post Raptor deletion. Flt3-Lin-Sca1+cKit+ cells were flow sorted from mice containing homozygous floxed alleles for exon 6 of the Raptor gene in the presence (MT group) or absence (WT group) of the MxCre transgene, which was induced with injections of mice with pIpC 5 weeks before cell isolation.
Experiment type
transcription profiling by array 
Contacts
Amit Sinha <amit.sinha@childrens.harvard.edu>, D Kalaitzidis, S A Armstrong
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-32265.idf.txt
Sample and data relationshipE-GEOD-32265.sdrf.txt
Raw data (1)E-GEOD-32265.raw.1.zip
Processed data (1)E-GEOD-32265.processed.1.zip
Array designA-AFFY-45.adf.txt
R ExpressionSetE-GEOD-32265.eSet.r
Links