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E-GEOD-32120 - The little elongation complex (LEC) regulates small nuclear RNA transcription

Status
Released on 22 December 2011, last updated on 2 June 2014
Organism
Drosophila melanogaster, Mus musculus
Samples (22)
Array (1)
Protocols (23)
Description
The Eleven-nineteen Lysine-rich Leukemia (ELL)-containing Super Elongation Complex (SEC) containing P-TEFb is a key regulator in the expression of HOX genes in Mixed Lineage Leukemia (MLL)-based leukemia. We have identified an SEC-like complex in Drosophila, as well as a distinct ELL-containing complex that lacks P-TEFb and other components of SEC named the "little elongation complex" (LEC). LEC subunits are highly enriched at RNA Polymerase II (Pol II) transcribed small nuclear RNA (snRNA) genes and the loss of LEC results in decreased snRNA expression in both flies and mammals. The discovery of specificity of SEC and LEC complexes for mRNA and snRNA containing genes, respectively, suggest the presence of specific classes of elongation factors for each class of genes transcribed by RNA polymerase II. Examination of genome-wide binding profiles for ELL, Ice1, Lilli, and Pol II in D. melanogaster and by ChIP-seq. Identification of differentially expressed genes in ELL-RNAi and Ice1-RNAi in D. melanogaster by RNA-seq. Examination of genome-wide binding profiles for ELL in M. musculus. Identification of differentially expressed genes in ELL-RNAi in M. musculus by RNA-seq.
Experiment types
ChIP-seq, transcription profiling by array, RNA-seq of coding RNA 
Contacts
Alexander (Garrett) Garruss <asg@stowers.org>, Alexander S Garrett, Ali Shilatifard, Edwin S Smith
Citation
The little elongation complex regulates small nuclear RNA transcription. Smith ER, Lin C, Garrett AS, Thornton J, Mohaghegh N, Hu D, Jackson J, Saraf A, Swanson SK, Seidel C, Florens L, Washburn MP, Eissenberg JC, Shilatifard A. , PMID:22195968
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-32120.idf.txt
Sample and data relationshipE-GEOD-32120.hyb.sdrf.txt, E-GEOD-32120.seq.sdrf.txt
Raw data (1)E-GEOD-32120.raw.1.zip
Processed data (19)Click to browse processed data
Array designA-AFFY-35.adf.txt
Links