E-GEOD-32084 - Transcriptomics of the traditional Japanese medicine juzentaihoto (JTX) on small intestines on germfree mice

Released on 13 September 2011, last updated on 2 May 2014
Mus musculus
Samples (12)
Array (1)
Protocols (8)
Juzehtaihoto, a Japanese traditional medicine has been used for the treatment of various kinds of disease or disorders in an enteric-flora dependent manner. Here, we performed transcriptome analysis using Affymetrix GeneChip on small intestine (SI) of germ-free (GF) and specific pathogen free (SPF) mice of IQI, an inbred strain established from ICR. Male germ-free (GF) and specific pathogen-free (SPF) IQI mice were bred and maintained in the laboratory of Central Institute for Experimental Animals (CIEA, Kawasaki, Kanagwa, Japan). GF mice were housed in a Trexler-type flexible film isolator in a standard germ free state and screened on a weekly basis for germ-free status by sampling feces sterilely and culturing on MRS-agar plates under aerobic and anaerobic conditions. All the GF, SPF, ex-GF mice were kept in a 12: 12-h light/dark cycle and at a temperature of 22±2°C. Nine week old mice (n=3) were sacrificed by cervical dislocation and the intestines were dissected. comparison of the mice with or without enteric flora
Experiment type
transcription profiling by array 
Kaori Munakata, Kenji Watanabe, Masahiro Yamamoto
Investigation descriptionE-GEOD-32084.idf.txt
Sample and data relationshipE-GEOD-32084.sdrf.txt
Raw data (1)E-GEOD-32084.raw.1.zip
Processed data (1)E-GEOD-32084.processed.1.zip
Array designA-AFFY-6.adf.txt