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E-GEOD-32078 - Global gene expression analysis of developing heart in embryos of diabetic mice

Status
Released on 13 September 2011, last updated on 29 September 2011
Organism
Mus musculus
Samples (12)
Array (1)
Protocols (7)
Description
Maternal diabetes causes cardiac malformations in fetuses. In this study, we have analyzed the differential gene expression profiling in the developing heart of embryos from diabetic and control mice by using the oligonucleotide microarray. Expression patterns of genes and proteins that are differentially expressed in the developing heart were further examined by the real-time reverse transcriptase-polymerase chain reaction and immunohistochemistry. Embryos of diabetic pregnancies displayed cardiac malformations. Microarray analysis revealed the genes that were altered in expression level in the developing heart of embryos from diabetic mice when compared to controls. It is concluded that altered expression of a variety of genes involved in heart development is associated with cardiac malformations in offsprings of diabetic mother. We used microarrays to identify the genes specific to the developing heart of embryos from control and diabetic mice RNA was isolated from heart tissue of control and diabetes exposed E13.5 and E15.5 mouse embryos (three samples each). The RNA was hybridised onto Affymetrix Mouse Genome 430 2.0 Array.
Experiment type
transcription profiling by array 
Contacts
Srinivasan Dinesh Kumar <antsdk@nus.edu.sg>, Dinesh K Srinivasan, Samuel S Tay
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-32078.idf.txt
Sample and data relationshipE-GEOD-32078.sdrf.txt
Raw data (1)E-GEOD-32078.raw.1.zip
Processed data (1)E-GEOD-32078.processed.1.zip
Array designA-AFFY-45.adf.txt
R ExpressionSetE-GEOD-32078.eSet.r
Links