E-GEOD-31665 - Role of Cofilin 1 pathway and actin dynamics in nuclear retinoid receptor function

Status
Released on 25 August 2011, last updated on 7 September 2011
Organism
Homo sapiens
Samples (12)
Array (1)
Protocols (10)
Description
We have investigated the role of actin dynamics and the effect of actin cytoskeleton modifying agents on retinoid receptor-mediated transactivation. Using Nef, an actin modifying HIV-1 protein, the role of LMK1/CFL1-mediated actin dynamics in receptor function was studied. The effect of Nef expression on transcriptome was investigated following transfection of HEK293 cells with Nef-expressing plasmid. The array data identified Nef-induced inhibition of a number of genes that contain retinoid receptor binding sites in their promoters. The experiment was designed to study the effect of expression of HIV-1 Nef protein on gene expression levels in HEK293 cells. Cells were transfected in three different experiments (each time in duplicate) with Nef expressing plasmid and a plasmid that contained a non-expressing Nef construct (Nef/Stop) as control. The cells were harvested after 36 of transfection and processed for gene array.
Experiment type
transcription profiling by array 
Contacts
Jun Yang <jyang@mail.nih.gov>, Mohammad Ishaq, Ven Natarajan
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-31665.idf.txt
Sample and data relationshipE-GEOD-31665.sdrf.txt
Raw data (2)E-GEOD-31665.raw.1.zip, E-GEOD-31665.raw.2.zip
Processed data (1)E-GEOD-31665.processed.1.zip
Array designA-AFFY-143.adf.txt
R ExpressionSetE-GEOD-31665.eSet.r
Links