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E-GEOD-31557 - Gene expression profile after injection of rhabdoviral recombinant protein NV

Status
Released on 11 August 2014, last updated on 16 August 2014
Organism
Oncorhynchus mykiss
Samples (16)
Array (1)
Protocols (15)
Description
Due to the common presence of a non-virion NV gene absent from other fish rhabdoviruses, NV-coding rhabdoviruses, such as the viral haemorrhagic septicemia (VHSV), were placed into a new Novirhabdovirus genus. Because conflicting results do exist on the importance of NV for VHSV trout pathogenicity and the NV function is still unclear, the effects of intraperitoneal injection of recombinant NV protein were studied by using home-designed immune-related microarrays from rainbow trout Oncorhynchus mykiss. The results were compared with parallel experiments using bacterial flagellins, a well know mammalian agonist of toll receptors (tlr5) with adjuvant activity recently described also in fish Four experimental groups were formed with four biological replicates each, 16 samples in total. First one was injected with NV recombinant protein in PBS, second one was a control group injected with PBS, the third group was injected with bacterial flagellins in PBS and the fourth group was injected with polihistidine peptide as a reference group because the recombinant proteins have a polihistidine tail. There is a technical replicate within each array.
Experiment type
transcription profiling by array 
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-31557.idf.txt
Sample and data relationshipE-GEOD-31557.sdrf.txt
Raw data (1)E-GEOD-31557.raw.1.zip
Processed data (1)E-GEOD-31557.processed.1.zip
Array designA-GEOD-14155.adf.txt
Links