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E-GEOD-31447 - Effect of endoplasmic reticulum stress on gene expression in lymphoblastoid cell lines

Status
Released on 22 August 2011, last updated on 6 September 2011
Organism
Homo sapiens
Samples (24)
Array (1)
Protocols (7)
Description
The ER stress inducing agent Thapsigargin (TG) and/or the cytoprotective agent Salubrinal were applied to lymphoblastoid cell lines. TG induced lytic replication as well as a distinct pattern of gene expression changes. This study was designed to identify host genes mediating lytic replication secondary to ER stress. DMSO or TG applied for 6 hrs then washed out, immediately followed by either (i) DMSO, or (ii) SAL resulting in 4 conditions (DMSO, TG, SAL, or TG+SAL). Total RNA harvested at 3, 24 or 72hrs for Affymetrix analysis.
Experiment type
transcription profiling by array 
Contacts
Adam Rosendorff
Citation
ER-stress causes Epstein-Barr virus lytic replication. Taylor GM, Raghuwanshi SK, Rowe DT, Wadowsky R, Rosendorff A. , PMID:21849482
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-31447.idf.txt
Sample and data relationshipE-GEOD-31447.sdrf.txt
Raw data (1)E-GEOD-31447.raw.1.zip
Processed data (1)E-GEOD-31447.processed.1.zip
Array designA-AFFY-44.adf.txt
R ExpressionSetE-GEOD-31447.eSet.r
Links