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E-GEOD-31369 - Transcription profiling by array of fission yeast rpb1-12XWTCTD and rpb1-12XS2ACTD strains

Status
Released on 31 August 2011, last updated on 26 January 2012
Organism
Schizosaccharomyces pombe
Samples (12)
Array (1)
Protocols (8)
Description
In fission yeast, the nuclear-localized Lsk1p-Lsc1p-Lsg1p cyclin dependent kinase complex is required for the reliable execution of cytokinesis and is also required for Ser-2 phosphorylation RNA pol II carboxy terminal domain. To address whether alterations in CTD phosphorylation might selectively alter expression of cytokinesis genes, expression profiling of site-directed CTD mutants was performed. Strains bearing the rpb1-12XCTD and rpb1-12XS2ACTD mutations were grown to mid-log phase in YES media and treated with 0.5uM LatA (or the solvent control, DMSO) for three hours at 30C. Three biological replicates were performed.
Experiment type
transcription profiling by array 
Contacts
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-31369.idf.txt
Sample and data relationshipE-GEOD-31369.sdrf.txt
Raw data (1)E-GEOD-31369.raw.1.zip
Processed data (1)E-GEOD-31369.processed.1.zip
Array designA-AFFY-47.adf.txt
R ExpressionSetE-GEOD-31369.eSet.r
Links