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E-GEOD-31288 - The impact of RNAi on the Saccharomyces cerevisiae transcriptome

Released on 10 August 2011, last updated on 3 April 2013
Saccharomyces cerevisiae
Samples (4)
Protocols (3)
The RNA interference (RNAi) pathway is found in most eukaryotic lineages but curiously is absent in others, including that of Saccharomyces cerevisiae. Here, we show that reconstituting RNAi in S. cerevisiae causes loss of a beneficial dsRNA virus, known as killer virus. Incompatibility between RNAi and killer viruses extends to other fungal species, in that RNAi is absent in all species known to possess dsRNA killer viruses, whereas killer viruses are absent in closely related species that retained RNAi. Thus, the advantage imparted by acquiring and retaining killer viruses explains the persistence of RNAi-deficient species during fungal evolution. Examine mRNA abundance of S. cerevisiae wild-type (DPB249), +AGO1 (DPB252), +DCR1 (DPB255) and +AGO1, DCR1 (DPB258).
Experiment type
RNA-seq of coding RNA 
Ines Anna Drinnenberg <>, D P Bartel, G R Fink, I A Drinnenberg
Exp. designProtocolsVariablesProcessedSeq. reads