E-GEOD-31288 - The impact of RNAi on the Saccharomyces cerevisiae transcriptome
Released on 10 August 2011, last updated on 3 April 2013
The RNA interference (RNAi) pathway is found in most eukaryotic lineages but curiously is absent in others, including that of Saccharomyces cerevisiae. Here, we show that reconstituting RNAi in S. cerevisiae causes loss of a beneficial dsRNA virus, known as killer virus. Incompatibility between RNAi and killer viruses extends to other fungal species, in that RNAi is absent in all species known to possess dsRNA killer viruses, whereas killer viruses are absent in closely related species that retained RNAi. Thus, the advantage imparted by acquiring and retaining killer viruses explains the persistence of RNAi-deficient species during fungal evolution. Examine mRNA abundance of S. cerevisiae wild-type (DPB249), +AGO1 (DPB252), +DCR1 (DPB255) and +AGO1, DCR1 (DPB258).
RNA-seq of coding RNA
Ines Anna Drinnenberg <firstname.lastname@example.org>, D P Bartel, G R Fink, I A Drinnenberg
Compatibility with killer explains the rise of RNAi-deficient fungi. Drinnenberg IA, Fink GR, Bartel DP. , PMID:21921191