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E-GEOD-31166 - Expression data from inducible ES stable cell line

Status
Released on 13 December 2012, last updated on 2 June 2014
Organism
Mus musculus
Samples (6)
Array (1)
Protocols (7)
Description
In order to identify the effects of the induction of the gene of interest on the mouse ES transcriptome, we performed Affymetrix Gene-Chip hybridization experiments for the inducible not-tagged cell line. Transcriptome analysis of the inducible transgenic mouse ES cell line. The E13 inducible cell lines derived from parental EB3 cell line. The cell line EB3 was obtained from the laboratory of Dr Hitoshi Niwa as previously described in (Masui S et al., 2005). Tthe specific mouse gene is E130012A19Rik. For the analysis on the inducible not-tagged cell line, total RNA was extracted from three biological replicates grown in medium deprived of Tetracycline for 48 hours; RNA extracted from un-induced clones was used as control.
Experiment type
transcription profiling by array 
Contacts
Rossella De Cegli
Citation
Reverse engineering a mouse embryonic stem cell-specific transcriptional network reveals a new modulator of neuronal differentiation. De Cegli R, Iacobacci S, Flore G, Gambardella G, Mao L, Cutillo L, Lauria M, Klose J, Illingworth E, Banfi S, di Bernardo D. , PMID:23180766
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-31166.idf.txt
Sample and data relationshipE-GEOD-31166.sdrf.txt
Raw data (1)E-GEOD-31166.raw.1.zip
Processed data (1)E-GEOD-31166.processed.1.zip
Array designA-AFFY-45.adf.txt
Links