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E-GEOD-31158 - Transcription profiling by array of Arabidopsis knocked out for or over expressing mpk6

Status
Released on 3 December 2011, last updated on 25 January 2012
Organism
Arabidopsis thaliana
Samples (25)
Array (1)
Protocols (8)
Description
MPK6 shows transient increase in activity under hypoxia with maximal activity at 2 hrs. To study the role of MPK6 in hypoxia in Arabidopsis, 10 do seedlings of WT, mpk6 and MPK6 plants were exposed to 2 hrs hypoxia and 2hr air (mock). Because there are no major phenotypic differences between the genotypes, microarray was used to determine if there were transcript level differences between the genotypes. Wild type Col-0, MPK6 overexpression and mpk6 knockout mutants were exposed to 2 hr hypoxia and air and separated into root and shoot in order to determine the role of MPK6 in hypoxia in both root and shoot.
Experiment type
transcription profiling by array 
Contacts
Charles Jang <cjang001@student.ucr.edu>, Cristina Branco-Price, Julia Bailey-Serres, Peter Nghiem, Ruth Chang
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-31158.idf.txt
Sample and data relationshipE-GEOD-31158.sdrf.txt
Raw data (1)E-GEOD-31158.raw.1.zip
Processed data (1)E-GEOD-31158.processed.1.zip
Array designA-AFFY-2.adf.txt
Links