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E-GEOD-30769 - Comparison of gene expression profiles in naïve WT and Asc-null bone marrow derived dendritic cells

Status
Released on 8 July 2012, last updated on 17 July 2012
Organism
Mus musculus
Samples (6)
Array (1)
Protocols (8)
Description
The adaptor protein ASC contributes to innate immunity through the assembly of caspase-1-activating inflammasome complexes. We demonstrate that ASC plays an inflammasome-independent cell-intrinsic role in adaptive immune cells. Asc-/- mice displayed defective antigen presentation by dendritic cells and lymphocyte migration due to impaired Rac-mediated actin polymerization. Genome-wide analysis showed that ASC, but not Nlrp3 or caspase-1, controls mRNA stability and expression of DOCK2, a guanine nucleotide exchange factor that mediates Rac-dependent signaling in immune cells. DOCK2-deficient dendritic cells showed similar defective antigen uptake as Asc-/- cells. Ectopic expression of DOCK2 in ASC-deficient cells restored Rac-mediated actin polymerization, antigen uptake and chemotaxis. Thus, ASC shapes adaptive immunity independently of inflammasomes by modulating DOCK2-dependent Rac activation and F-actin polymerization in dendritic cells and lymphocytes. Three replicates of naïve WT and three replicates of Asc-/- bone marrow derived dendritic cells were analyzed on the Affymetrix HT MG-430 PM plate array.
Experiment type
transcription profiling by array 
Contacts
Geoffrey Neale <geoffrey.neale@stjude.org>, Geoffrey A Neale, Patrick J Shaw, R K Malireddi, Sirish K Ippagunta, Thirumala-Devi Kanneganti
Citation
The inflammasome adaptor ASC regulates the function of adaptive immune cells by controlling Dock2-mediated Rac activation and actin polymerization. Ippagunta SK, Malireddi RK, Shaw PJ, Neale GA, Walle LV, Green DR, Fukui Y, Lamkanfi M, Kanneganti TD. , PMID:21892172
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-30769.idf.txt
Sample and data relationshipE-GEOD-30769.sdrf.txt
Raw data (1)E-GEOD-30769.raw.1.zip
Processed data (1)E-GEOD-30769.processed.1.zip
Array designA-GEOD-11180.adf.txt
R ExpressionSetE-GEOD-30769.eSet.r
Links