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E-GEOD-30436 - Transcriptome profiling of reproductive stage flag leaves of wheat from drought susceptible parent WL711, drought tolerant parent C306 and drought susceptible and drought tolerant RIL bulks in irrigated and drought condition

Status
Released on 29 April 2012, last updated on 25 June 2012
Organism
Triticum aestivum
Samples (24)
Array (1)
Protocols (6)
Description
The aim of the study was to identify candidate genes responsible for drought tolerance trait between a pair of wheat varieties ( WL711 and C306) and correspondng progeny bulks (10 drought susceptible RILs and 10 drought tolerant RILs) by combining QTLs analysis with expression analysis. Microarray analysis of RNA extracted from the flag leaves showed large number of differentially expressed genes. The number of differentially expressed genes was reduced to 37 on the basis of their occurance in a major QTL region (responcible for drought tolerance) detected in RIL population derived from WL711 and C306. RNA from the reproductive stage flag leaf of wheat in irrigated and drought condition of drought susceptible parent WL711, drought tolerant parent C306 and drought susceptible and drought tolerant RIL bulks were analysed in three different biological replications (A , B and C) making total twenty four samples
Experiment type
transcription profiling by array 
Contacts
suhas baliram kadam <suhaskad@gmail.com>, Kishor Gaikwad, Nagendra K Singh, Renu K Chopra, Suhas B Kadam, Vasantrao N Pawar
Citation
Genomic associations for drought tolerance on the short arm of wheat chromosome 4B. Kadam S, Singh K, Shukla S, Goel S, Vikram P, Pawar V, Gaikwad K, Khanna-Chopra R, Singh N. , PMID:22476619
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-30436.idf.txt
Sample and data relationshipE-GEOD-30436.sdrf.txt
Raw data (1)E-GEOD-30436.raw.1.zip
Processed data (1)E-GEOD-30436.processed.1.zip
Array designA-AFFY-57.adf.txt
R ExpressionSetE-GEOD-30436.eSet.r
Links