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E-GEOD-30405 - The ets transcription factor ELF5 suppresses the estrogen sensitive phenotype and contributes to antiestrogen resistance in luminal breast cancer. [human]

Status
Released on 27 December 2012, last updated on 14 January 2013
Organism
Homo sapiens
Samples (14)
Array (1)
Protocols (10)
Description
The ets transcription factor ELF5 specifies the differentiation of mammary progenitor cells to establish the milk-secreting lineage. ER- and poor prognosis basal breast cancers arise from this progenitor cell and these cancers express high levels of Elf5. Knockdown of ELF5 expression in basal breast cancer cell lines, or forced expression in luminal breast cancer cell lines, resulted in reduced cell proliferation. Transcript profiling and chromatin immunoprecipitation revealed that the transcriptional activity of ELF5 specified the gene expression patterns that distinguish basal from luminal breast cancer, including suppression of FOXA1, GATA3 and ER, key estrogen-action genes. Tamoxifen treatment of luminal MCF7 cells upregulated Elf5 expression and cells that acquired resistance to Tamoxifen became dependent on ELF5 for proliferation. ELF5 is a regulator of breast cancer cell proliferation, transcriptionally specifies the basal molecular subtype and is utilised by ER+ breast cancer cells to escape proliferative arrest caused by Tamoxifen. Elf5 was knocked down via siRNA in basal HCC1937 cell lines, in triplicate. Elf5 was induced in luminal T47D and MCF7 cell lines via a doxycycline inducible expression vector, in duplicate.
Experiment type
transcription profiling by array 
Contacts
Mark Cowley <m.cowley@garvan.org.au>, Aaron Statham, Adelaide Young, Alex Swarbrick, Andrea Egger, Andrew Stone, Cara Evans, Catherine Piggin, Chehani Alles, Christopher J Ormandy, David Gallego-Ortega, Fatima Valdes-Mora, Heather Lee, Jason Carroll, Julia M Gee, Liz Caldon, Maria Kalyuga, Mark J Cowley, Matthew J Naylor, Nicola Armstrong, Robert I Nicholson, Robert L Sutherland, Rosalind Launchbury, Stephanie Allerdice, Susan Clark, Tilman Brummer, Warren Kaplan, Wendy Au
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-30405.idf.txt
Sample and data relationshipE-GEOD-30405.sdrf.txt
Raw data (1)E-GEOD-30405.raw.1.zip
Processed data (1)E-GEOD-30405.processed.1.zip
Array designA-AFFY-141.adf.txt
Links