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E-GEOD-30331 - Global gene expression analysis of Yersinia pestis AHL quorum sensing at 37°C

Status
Released on 3 July 2016, last updated on 28 December 2016
Organism
Yersinia pestis
Samples (12)
Array (1)
Protocols (7)
Description
Quorum sensing is a cell to cell communication process that involves chemical signaling. Yersinia pestis, the agent of plague, has two functional AHL quorum sensing systems Ysp and Ype. For several reasons, it was not clear what effect AHL pathways have on virulence gene expression and survival in the two different hosts, flea and human. To investigate to what effect AHL quorum sensing has on gene expression, we conducted microarray studies comparing Yersinia pestis CO92 (∆pgm) to a double AHL mutant strain (∆pgm ΔypeIR ΔyspIR) at 37°C. Six independent RNA samples from Y. pestis CO92 R114 AHL deficient cultures were paired with six independent RNA samples from control Y. pestis CO92 R88 cultures for hybridization to six two-color microarrays. For three arrays, the control RNA sample was labeled with Alexa 555 dye and the experimental RNA sample was labeled with Alexa 647 dye; the dyes were reversed for the other three arrays to account for any dye bias.
Experiment type
transcription profiling by array 
Contacts
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-30331.idf.txt
Sample and data relationshipE-GEOD-30331.sdrf.txt
Raw data (1)E-GEOD-30331.raw.1.zip
Processed data (1)E-GEOD-30331.processed.1.zip
Array designA-GEOD-9009.adf.txt
Links