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E-GEOD-30096 - Transcription profiling by array of Arabidopsis roots after growth in low pH media

Status
Released on 23 November 2011, last updated on 22 August 2014
Organism
Arabidopsis thaliana
Samples (16)
Array (1)
Protocols (8)
Description
To understand the effect of low pH on developmental stages in the root, we dissected the root into four developmental zones after exposure to low pH and expression profiled each zone. Stress responses in plants are tightly coordinated with developmental processes, but the interaction between these pathways is poorly understood. Here we use genome-wide assays at high spatial and temporal resolution to understand the processes that lnk development and stress in the Arabidopsis root. Our meta-analysis finds little evidence for a universal stress response. Common stress responses appear to exists and, analagous to animal systems, many of them show cell-type specificity, suggesting a convergent evolutionary theme in multicellular organisms. Common stress responses may be mediated by cell identity regulators, as mutations in these genes resulted in altered responses to stress. Our results reveal surprising linkages between stress and development at cellular resolution, and show the power of multiple genome-wide datasets to elucidate biological processes. 2 replicates each of 4 developmental stages exposed to standard and low pH
Experiment type
transcription profiling by array 
Contacts
Anjali S Iyer-Pascuzzi, Philip N Benfey
Citation
Cell identity regulators link development and stress responses in the Arabidopsis root. Iyer-Pascuzzi AS, Jackson T, Cui H, Petricka JJ, Busch W, Tsukagoshi H, Benfey PN. , PMID:22014526
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-30096.idf.txt
Sample and data relationshipE-GEOD-30096.sdrf.txt
Raw data (1)E-GEOD-30096.raw.1.zip
Processed data (1)E-GEOD-30096.processed.1.zip
Array designA-AFFY-2.adf.txt
Links