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E-GEOD-29946 - Genome wide transcriptional analysis of P. aeruginosa PAO1 response to kappa-opioid U-50,488 in poor nutrient medium

Status
Released on 20 May 2012, last updated on 31 May 2012
Organism
Pseudomonas aeruginosa PAO1
Samples (12)
Array (1)
Protocols (7)
Description
Here we examined virulence activation in Pseudomonas aeruginosa in response to the synthetic kappa opioid agonist U-50, 488 in nutrient poor media where growth conditions are limited and density dependent quorum sensing is not activated. We used a microarray to define the virulence-related genes in P. aeruginosa grown in liquid poor nutrient medium to determine the effect of kappa-opioid receptor agonist on virulence/lethal phenotype All biological samples for gene expression analysis were prepared in triplicate. P. aeruginosa MPAO1 cells collected from liquid culture at 7 hrs of growth in 1.) 0.1xTY medium containing tryptone 1 g/L and yeast extract, 0.5 g/L, or 2.) 0.1xTY+25 mM potassium phosphate buffer, pH 6.0, or 3.) 0.1xTY+200 uM U-50,488, or 4.) 0.1xTY+ 25 mM potassium phosphate buffer, pH 6.0+ 200 uM U-50,488 were used for RNA isolation. Microarray analysis was performed using Affymetrix P. aeruginosa GeneChips (Affymetrix, Santa Clara, CA) at the University of Chicago Functional Genomics Facility
Experiment type
transcription profiling by array 
Contacts
Citation
The use of fluoroquinolones in chronic otitis suppurativa. van de Heyning PH, Pattyn SR, Valcke HD, van Caekenberghe DL, Claes J.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-29946.idf.txt
Sample and data relationshipE-GEOD-29946.sdrf.txt
Raw data (1)E-GEOD-29946.raw.1.zip
Processed data (1)E-GEOD-29946.processed.1.zip
Array designA-AFFY-30.adf.txt
Links