Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.

E-GEOD-29740 - Molecular characterization of the incompatible and compatible interaction of soybean rust disease in Rpp3-containing PI462312 line

Status
Released on 28 November 2011, last updated on 26 June 2012
Organism
Glycine max
Samples (54)
Array (1)
Protocols (6)
Description
Experimental design: 1 genotype: PI- (resistant USDA Plant Introduction (PI462312) line containing SBR Rpp3 resistance gene) 3 treatments: Virulent soybean rust (Phakopsora pachyrhizi Tw80-2) challenge, avirulent soybean rust challenge (Hw94-1) & mock infection 3 replications 6 time points: 12, 24, 72, 144, 216 and 288 hours after inoculation TOTAL: 54 Affymetrix GeneChip(R) Soybean Genome Arrays Mock treatment: 0.01% Tween 20 Hawaii 94 treatment: 500,000 spores per ml in 0.01% Tween 20 Taiwan 80-2 treatment: 500,000 spores per ml in 0.01% Tween 20 ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Martijn van de Mortel (for Steve Whitham). The equivalent experiment is GM36 at PLEXdb.] pathogen isolates: Mock - time: 12 hai(3-replications); pathogen isolates: Mock - time: 24 hai(3-replications); pathogen isolates: Mock - time: 72 hai(3-replications); pathogen isolates: Mock - time: 144 hai(3-replications); pathogen isolates: Mock - time: 216 hai(3-replications); pathogen isolates: Mock - time: 288 hai(3-replications); pathogen isolates: Taiwan 80-2 (vir) - time: 12 hai(3-replications); pathogen isolates: Taiwan 80-2 (vir) - time: 24 hai(3-replications); pathogen isolates: Taiwan 80-2 (vir) - time: 72 hai(3-replications); pathogen isolates: Taiwan 80-2 (vir) - time: 144 hai(3-replications); pathogen isolates: Taiwan 80-2 (vir) - time: 216 hai(3-replications); pathogen isolates: Taiwan 80-2 (vir) - time: 288 hai(3-replications); pathogen isolates: Hawaii 94-1 (avir) - time: 12 hai(3-replications); pathogen isolates: Hawaii 94-1 (avir) - time: 24 hai(3-replications); pathogen isolates: Hawaii 94-1 (avir) - time: 72 hai(3-replications); pathogen isolates: Hawaii 94-1 (avir) - time: 144 hai(3-replications); pathogen isolates: Hawaii 94-1 (avir) - time: 216 hai(3-replications); pathogen isolates: Hawaii 94-1 (avir) - time: 288 hai(3-replications)
Experiment type
transcription profiling by array 
Contacts
Citation
Biphasic gene expression changes elicited by Phakopsora pachyrhizi in soybean correlate with fungal penetration and haustoria formation. Schneider KT, van de Mortel M, Bancroft TJ, Braun E, Nettleton D, Nelson RT, Frederick RD, Baum TJ, Graham MA, Whitham SA. , PMID:21791600
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-29740.idf.txt
Sample and data relationshipE-GEOD-29740.sdrf.txt
Raw data (2)E-GEOD-29740.raw.1.zip, E-GEOD-29740.raw.2.zip
Processed data (1)E-GEOD-29740.processed.1.zip
Array designA-AFFY-59.adf.txt
Links