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E-GEOD-29661 - Cellular aging can be monitored by DNA-methylation changes at specific CpG sites
Released on 21 May 2012, last updated on 31 May 2012
Long-term culture associated changes need to be considered for quality control of cell preparations – especially in cellular therapy. Here we describe a simple method to track cellular aging based on continuous DNA-methylation changes at six specific CpG sites. This epigenetic signature can be used as a biomarker for various cell types to predict the state of cellular aging with regard to the number of passages or days of in vitro culture. 8 samples of human dermal fibroblasts. 4 samples of mesenchymal stem cells (MSC) from human adipose tissue.
methylation profiling by array
Wolfgang Wagner <firstname.lastname@example.org>, Anne Schellenberg, Carmen M Koch, Martin Zenke, Qiong Lin, Sylvia Joussen
Monitoring of cellular senescence by DNA-methylation at specific CpG sites. Koch CM, Joussen S, Schellenberg A, Lin Q, Zenke M, Wagner W. , PMID:22221451