Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.

E-GEOD-29631 - M. smegmatis vapBC::aphA-3 ΔvapBC mutant (JR121) harboring tetracycline inducible vector pMind containing the vapBC operon (pJR230) and the pMind vector containing the vapC toxin gene (pJR29)

Status
Released on 14 June 2012, last updated on 3 May 2014
Organism
Mycobacterium smegmatis
Samples (8)
Array (1)
Protocols (7)
Description
Transcriptional profiling of M. smegmatis JR121 expressing VapC and VapBC grown in flasks on Hartmans de Bont medium supplemented with 0.2% glycerol Comparing transcriptional response of strain JR121 to conditional expression of VapC toxin compared with the expression of VapBC complex. Biological replicates: 4 independently grown and harvested. One replicate per array.
Experiment type
transcription profiling by array 
Contacts
Jennifer R Robson <jenrobson1@gmail.com>, Gregory M Cook, Jennifer Robson, Joanna L McKenzie, Vickery L Arcus
Citation
A VapBC toxin-antitoxin module is a posttranscriptional regulator of metabolic flux in mycobacteria. McKenzie JL, Robson J, Berney M, Smith TC, Ruthe A, Gardner PP, Arcus VL, Cook GM. , PMID:22366418
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-29631.idf.txt
Sample and data relationshipE-GEOD-29631.sdrf.txt
Raw data (1)E-GEOD-29631.raw.1.zip
Processed data (1)E-GEOD-29631.processed.1.zip
Array designA-GEOD-13665.adf.txt
Links