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E-GEOD-28833 - The Polycomb group mutant esc leads to augmented the levels of paused Pol II in the Drosophila embryo

Status
Released on 14 June 2011, last updated on 2 June 2014
Organism
Drosophila melanogaster
Samples (18)
Protocols (7)
Description
The role of Polycomb group (PcG) was studied on RNA Polymerase II (Pol II) pausing phenomenon. Wild type and Polycomb mutant (esc) embryos were used for ChIP-Seq experiments using Pol II and histone methylation antibodies. Enhanced Pol II occupancy was observed for thousands of genes in esc mutant embryos, including genes not known to be bound by PRC2 or having H3K27me3 associated. Most of these genes exhibit a concomitant reduction in H3K27me3 and increase in H3K4me3 at promoter-proximal regions. Silent genes lacking promoter-associated paused Pol II in wild-type embryos are converted into “poised” genes with paused Pol II in esc mutants. We suggest that this conversion of silent genes into poised genes might render differentiated cell types susceptible to switches in identity in PcG mutants. ChIP-Seq data was generated for antibodies against Pol-II, H3K4me3, H3K27me3 in both wild type and esc mutant Drosophila embryos (0-2hr, 8-12hr, and 18-24hr embryos). In addition GRO-Seq data was generated for 18-24hr esc mutant embryos.
Experiment types
ChIP-seq, RNA-seq of coding RNA 
Contacts
David Anthony Hendrix <davehendrix@gmail.com>, Chiahao Tsui, David A Hendrix, John T Lis, Leighton J Core, Michael Levine, Vivek S Chopra
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-28833.idf.txt
Sample and data relationshipE-GEOD-28833.sdrf.txt
Processed data (18)Click to browse processed data
Links