E-GEOD-2882 - Transcription profiling of microdissected and hand sorted mouse forebrain neurons to understand the extend and nature of differential expression in various cell types
Submitted on 5 July 2005, released on 31 August 2007, last updated on 27 March 2012
The mammalian forebrain is a tissue of stunning complexity comprised of numerous regions each containing many distinct cell types that differ in their intrinsic and synaptic physiology, morphology and connectivity. These differences are likely conferred by differential gene expression, but the extent and nature of cell type specific gene expression is largely unknown. Here, we carried out microarray analysis of twelve major classes of fluorescently labelled neurons within the forebrain and provide the first comprehensive view of gene expression differences. The results demonstrate a profound molecular heterogeneity among neuronal subtypes, represented disproportionately by gene paralogs, and begin to reveal the genetic programs underlying the fundamental divisions between neuronal classes including that between glutamatergic and GABAergic neurons. Experiment Overall Design: Total of 36 samples from 12 different cell types were anlyzed. Three biological replicates (each sample from different animal(s)) were analyzed for each cell type.
transcription profiling by array, cell type comparison, co-expression, organism part comparison
Molecular taxonomy of major neuronal classes in the adult mouse forebrain. Ken Sugino, Chris M Hempel, Mark N Miller, Alexis M Hattox, Peter Shapiro, Caizi Wu, Z Josh Huang, Sacha B Nelson. Nat Neurosci 9(1):99-107 (2006)