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E-GEOD-28741 - sncRNAs in E. faecalis V538

Status
Released on 21 April 2011, last updated on 3 May 2014
Organism
Enterococcus faecalis V583
Samples (2)
Array (1)
Protocols (8)
Description
Small non coding RNA molecules (sncRNAs) are key mediators of virulence and stress inducible gene expressions in some pathogens. In this work we identify sncRNAs in the Gram positive opportunistic pathogen Enterococcus faecalis. Enterococcus faecalis. We characterized 11 sncRNAs by tiling microarray analysis, 5’ and 3’ RACE-PCR, and Northern blot analysis. Six sncRNAs were specifically expressed at exponential phase, two sncRNAs were observed at stationary phase, and three were detected during both phases. This is the first experimental genome-wide identification of sncRNAs in E. faecalis and provides impetus to the understanding of gene regulation in this important human pathogen. Identification of sncRNA candidates transcribed by E. faecalis V583 was undertaken with two samples of cells harvested in mid-log growth phase and stationary phase after 24h of incubation at 37°C in M17 glucose media.
Experiment type
transcription profiling by array 
Contacts
jean-christophe giard <jean-christophe.giard@unicaen.fr>, Axel Hartke, Carsten Kuenne, Charlotte Michaux, Jean-Christophe Giard, Kouki Shioya, Nicolas Verneuil, Thomas Hartsch, Torsten Hain
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-28741.idf.txt
Sample and data relationshipE-GEOD-28741.sdrf.txt
Raw data (1)E-GEOD-28741.raw.1.zip
Processed data (1)E-GEOD-28741.processed.1.zip
Array designA-GEOD-13432.adf.txt
Links