E-GEOD-28453 - Dynamics of Sox2 occupancy during the differentiation of embryonic stem cells into trophoblast stem cells
Released on 17 October 2013, last updated on 24 February 2015
To understand the mechanism underlying the versatility in transcriptional regulation by Sox2, we compared genome-wide binding sites of Sox2 in embryonic stem (ES) cells and trophoblast stem (TS) cells by chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq). A tetracycline-inducible Oct3/4 knockout ES cell line ZHBTc4 was treated with Tet for 4 days in the presence of FGF4 and mouse embryonic fibroblasts (MEFs).
Itoshi NIKAIDO <email@example.com>, Hiroki R Ueda, Hitoshi Niwa, Itoshi Nikaido, Kenjiro Adachi
Context-Dependent Wiring of Sox2 Regulatory Networks for Self-Renewal of Embryonic and Trophoblast Stem Cells. Adachi K, Nikaido I, Ohta H, Ohtsuka S, Ura H, Kadota M, Wakayama T, Ueda HR, Niwa H. , Europe PMC 24120664