Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.
E-GEOD-28135 - PRMT1 interacts with leukemia fusion protein AML1-ETO for activation of transcription and transformation
Released on 17 June 2012, last updated on 26 June 2012
Fusion protein AML1-ETO resulted from t(8;21) translocation is highly related to leukemia development. We have previously shown that the expression of AE9a, a spliced form of AML1-ETO, can rapidly cause leukemia in mouse. To understand how AML1-ETO is involved in leukemia development, we used AE9a leukemia model to identify a novel AE9a interacting proteins PRMT1 (protein arginine methyltransferase 1) from primary leukemic cells expressing AE9a. To examine whether PRMT1 is involved in AE9a-mediated transcription regulation, genome wide gene expression analysis is carried out in hematopoietic cell line K562 (wild type or AE9a expressing) treated with (-) control siRNA or siPRMT1. Wild type or AE9a-expressing K562 cells with control siRNA or siPRMT1 in triplicate
transcription profiling by array
Dong-Er Zhang <firstname.lastname@example.org>, Akiko J Okumura, Ali Sarkeshik, John R Yates III, Miao-Chia Lo, Ming Yan, Shinobu Matsuura, Stephen D Nimer, Wei-Jong Shia, Xinyang Zhao
PRMT1 interacts with AML1-ETO to promote its transcriptional activation and progenitor cell proliferative potential. Shia WJ, Okumura AJ, Yan M, Sarkeshik A, Lo MC, Matsuura S, Komeno Y, Zhao X, Nimer SD, Yates JR 3rd, Zhang DE. , PMID:22498736