6 protocols
AccessionType
bioassay_data_transformation
ID_REF =
VALUE = RMA summarized expression value
feature_extraction
RMA normalization was used to summarize the signal using Partek Genome Suite 6.5.
image_aquisition
Standard Affymetrix protocol.
hybridization
Standard Affymetrix protocol.
labeling
According to the MessageAmp™ II-Biotin Enhanced Kit protocol (Ambion).
nucleic_acid_extraction
Total RNA was extracted from four eyes from four 8-week-old C57B/6 mice using the mirVana RNA isolation kit (Ambion) and pooled together.