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E-GEOD-27404 - Deep sequencing and functional annotation reveal miRNAs implicated in the cell senescence phenotype

Status
Released on 9 August 2011, last updated on 22 August 2013
Organism
Homo sapiens
Samples (2)
Protocols (4)
Description
In cell senescence, cultured cells cease proliferating and acquire aberrant gene expression patterns. MicroRNAs (miRNAs) modulate gene expression through translational repression or mRNA degradation, and have been implicated in senescence. We have used deep sequencing to carry out a comprehensive survey of miRNA expression and its involvement in cell senescence. Informatic analysis of small RNA sequence datasets from young and senescent IMR90 human fibroblasts identifies many known miRNAs, and a small number of novel miRNAs, that are regulated (either up or down) with cell senescence. Comparison with mRNA expression profiles revealed potential mRNA targets of the senescence-regulated miRNAs. The target mRNAs are enriched for genes involved in biological processes associated with cell senescence. This result greatly extends existing information on the role of miRNAs in cell senescence, and is consistent with miRNAs having a causal role in the process. Comprehensive survey of miRNA from young and senescent IMR90 fibroblasts using deep sequencing
Experiment type
RNA-seq of non coding RNA 
Contacts
Dario Boffelli, Joseph Dhahbi
Citation
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-27404.idf.txt
Sample and data relationshipE-GEOD-27404.sdrf.txt
Processed data (1)E-GEOD-27404.processed.1.zip
Links