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E-GEOD-27402 - Expression data from WT, HEB-KO and E2A-KO LY6D- CLP cells

Status
Released on 22 November 2011, last updated on 6 December 2011
Organism
Mus musculus
Samples (8)
Array (1)
Protocols (6)
Description
The E-protein transcription factors E2A and HEB play important roles at several stages of hematopoiesis. However, the exact mechanism for theire action and the main targets in the LY6D negative common lymphoid progentior (CLP) compartment remains unknown. By adressing this question, we will gain important infromation regarding the early events leading to B-cell specification. FACS sorted LY6D negative common lymphoid progenitors from WT, HEB-KO and E2A-KO mice were subjected to RNA extraction and hybridization on Affymetrix microarrays. At least two independent sorts were performed per genotype. During each sort, cells were pooled from several bone marrows.
Experiment type
transcription profiling by array 
Contacts
Eva Welinder <ewelinder@gmail.com>, Cornelis Murre, David Bryder, Elinore Mercer, Mikael Sigvardsson, Robert Mansson
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-27402.idf.txt
Sample and data relationshipE-GEOD-27402.sdrf.txt
Raw data (1)E-GEOD-27402.raw.1.zip
Processed data (1)E-GEOD-27402.processed.1.zip
Array designA-AFFY-45.adf.txt
R ExpressionSetE-GEOD-27402.eSet.r
Links