E-GEOD-26862 - Expression data of human eGFP-SOX17+ endoderm cells
Status | Released on 15 March 2011, last updated on 2 June 2014 | ||||||||||
Organism | Homo sapiens | ||||||||||
Samples (9) | |||||||||||
Array (1) | |||||||||||
Protocols (8) | |||||||||||
Description | Using homologous recombination in human ESC, we inserted an enhanced green fluorescent protein (eGFP) transgene into a locus encoding a postulated marker of human endoderm, SOX17 in H9 human embryonic stem cells. This allowed purification of SOX17+ hESC endodermal progeny by fluorescence activated cell sorting (FACS) to generate microarray gene expression profile. Using Wnt3 and Activin to differentiate hSOX17-2 to stage 1 cells, and subsequently FGF10 and cyclopamine to stage 2 cells, we isolated eGFP+ cells by FACS at each stage, performed microarray analysis. | ||||||||||
Experiment type | transcription profiling by array | ||||||||||
Contacts | Kim Seung, Pei Wang | ||||||||||
Citation | Targeting SOX17 in human embryonic stem cells creates unique strategies for isolating and analyzing developing endoderm. Wang P, Rodriguez RT, Wang J, Ghodasara A, Kim SK. , PMID:21362573 | ||||||||||
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