E-GEOD-26862 - Expression data of human eGFP-SOX17+ endoderm cells

Status
Released on 15 March 2011, last updated on 2 June 2014
Organism
Homo sapiens
Samples (9)
Array (1)
Protocols (8)
Description
Using homologous recombination in human ESC, we inserted an enhanced green fluorescent protein (eGFP) transgene into a locus encoding a postulated marker of human endoderm, SOX17 in H9 human embryonic stem cells. This allowed purification of SOX17+ hESC endodermal progeny by fluorescence activated cell sorting (FACS) to generate microarray gene expression profile. Using Wnt3 and Activin to differentiate hSOX17-2 to stage 1 cells, and subsequently FGF10 and cyclopamine to stage 2 cells, we isolated eGFP+ cells by FACS at each stage, performed microarray analysis.
Experiment type
transcription profiling by array 
Contacts
Kim Seung, Pei Wang
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-26862.idf.txt
Sample and data relationshipE-GEOD-26862.sdrf.txt
Raw data (1)E-GEOD-26862.raw.1.zip
Processed data (1)E-GEOD-26862.processed.1.zip
Array designA-AFFY-44.adf.txt
Links