E-GEOD-26862 - Expression data of human eGFP-SOX17+ endoderm cells
Released on 15 March 2011, last updated on 2 June 2014
Using homologous recombination in human ESC, we inserted an enhanced green fluorescent protein (eGFP) transgene into a locus encoding a postulated marker of human endoderm, SOX17 in H9 human embryonic stem cells. This allowed purification of SOX17+ hESC endodermal progeny by fluorescence activated cell sorting (FACS) to generate microarray gene expression profile. Using Wnt3 and Activin to differentiate hSOX17-2 to stage 1 cells, and subsequently FGF10 and cyclopamine to stage 2 cells, we isolated eGFP+ cells by FACS at each stage, performed microarray analysis.
transcription profiling by array
Kim Seung, Pei Wang
Targeting SOX17 in human embryonic stem cells creates unique strategies for isolating and analyzing developing endoderm. Wang P, Rodriguez RT, Wang J, Ghodasara A, Kim SK. , PMID:21362573