8 protocols
AccessionType
bioassay_data_transformation
ID_REF =
VALUE = RMA signal intensity
feature_extraction
RMA processing of CEL files, standard Affymetrix CDF.
labeling
Standard Affymetrix labeling protocol to generate biotinylated cRNA applied to 2ug total RNA for MOE 430 plus 2.0 GeneChip.
nucleic_acid_extraction
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
image_aquisition
GeneChips were scanned using the Affymetrix GeneArray Scanner.
hybridization
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse MOE 430 Plus 2.0 Genome Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
grow
Microisolator housed mice in clean mouse facility.
specified_biomaterial_action
Mice were treated with 2-3 micrograms of IL-13, intratracheally 1x/day x 7 days. Lungs were collected 24 hours after final administration.