VALUE = RMA signal intensity
RMA processing of CEL files, standard Affymetrix CDF.
Standard Affymetrix labeling protocol to generate biotinylated cRNA applied to 2ug total RNA for MOE 430 plus 2.0 GeneChip.
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
GeneChips were scanned using the Affymetrix GeneArray Scanner.
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse MOE 430 Plus 2.0 Genome Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Microisolator housed mice in clean mouse facility.
Mice were treated with 2-3 micrograms of IL-13, intratracheally 1x/day x 7 days. Lungs were collected 24 hours after final administration.