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E-GEOD-26401 - Study of mating type genes and their roles in mating interactions of the basidiomycete Schizophyllum commune

Status
Released on 14 June 2012, last updated on 26 June 2012
Organism
Schizophyllum commune
Samples (16)
Array (1)
Protocols (8)
Description
Transcriptome changes associated with mating interactions were performed in order to identify signaling components and targets of pheromone response. Two monokaryons (S. commune 12-43 and 4-39), a dikaryon (W22 x 12-43), both semi-compatible mating interactions (Aon: W22 x 4-39; Bon, flat: W21 x 4-39), and different S. commune pheromone receptor recipient strains (Vbar2f, Vbar2t) were analyzed for that purpose. In addition, a S. commune strain showing the thin-phenotype (W22-thin) was investigated to figure out the role of Thn1 in mating. With microarray analysis mRNA expression in different mating interactions of the basidiomycete S. commune was analyzed. Genes which show regulation in the comparison of the two monokaryons 12-43 and 4-39 has been eliminated from other comparisons as strain specific differences. Two biological or technical replicate samples were analyzed for each condition.
Experiment type
transcription profiling by array 
Contacts
Susann Erdmann <susann.erdmann@uni-jena.de>, Daniela Freihorst, Erika Kothe
Citation
Transcriptome and functional analysis of mating in the basidiomycete Schizophyllum commune. Erdmann S, Freihorst D, Raudaskoski M, Schmidt-Heck W, Jung EM, Senftleben D, Kothe E. , PMID:22210832
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-26401.idf.txt
Sample and data relationshipE-GEOD-26401.sdrf.txt
Processed data (1)E-GEOD-26401.processed.1.zip
Additional data (1)E-GEOD-26401.additional.1.zip
Array designA-GEOD-11376.adf.txt
Links