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E-GEOD-26315 - Expression data from human amnion mesenchymal cells treated with interleukin-1-beta for 1hr and 8hr

Status
Released on 2 June 2011, last updated on 17 June 2011
Organism
Homo sapiens
Samples (9)
Array (1)
Protocols (8)
Description
Premature birth continues to be a challenging pregnancy complication, and a body of literature indicates that inflammation can contribute to premature delivery by converting a receptive uterine environment to a hostile one. Cytokines have been demonstrated to provoke up-regulation of inflammatory genes (e.g. interleukin-1, 6, and 8, tumor necrosis factor-alpha, cyclooxygenase-2, and microsomal prostaglandin E synthase-1). Using monolayer cultures of human amnion mesenchymal cells (AMCs) as a model, we aimed to detail the global programme of gene expression that occurs in response to cytokine challenge. Human amnion mesenchymal cells were challenged with 10 ng/ml of interleukin-1-beta for 1 hour (1h) or 8 hours (8h) in serum-free media, while control cells were treated with serum free medium only (veh). The cells (veh, 1h and 8h) were subjected to RNA extraction, quantification, and evaluation, followed by hybridization on Affymetrix GeneChip® Human Genome U133A 2.0 Arrays (Platform GPL571). Experiments were repeated three times for a total of nine samples used for microarray analysis. Three biological replicates were used to ensure that any results were not biased by day-to-day variation in RNA extraction.
Experiment type
transcription profiling by array 
Contacts
Douglas A. Kniss <geo@ncbi.nlm.nih.gov>, Douglas A Kniss, Jie Zhang, Kun Huang, Lianbo Yu, Parul Galati, Roberto Romero, Ruth Li, Taryn L Summerfield, William E Ackerman IV
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-26315.idf.txt
Sample and data relationshipE-GEOD-26315.sdrf.txt
Raw data (1)E-GEOD-26315.raw.1.zip
Processed data (1)E-GEOD-26315.processed.1.zip
Array designA-AFFY-37.adf.txt
R ExpressionSetE-GEOD-26315.eSet.r
Links