Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.

E-GEOD-26266 - Transcription profiling by array of Arabidopsis Col-0 and Ws ecotypes and QK mutants after heat-shock treatment

Status
Released on 23 December 2010, last updated on 26 January 2012
Organism
Arabidopsis thaliana
Samples (12)
Array (1)
Protocols (8)
Description
In order to study the importance of HSFA1 in thermotolerance in Arabidopsis, we generated the HSFA1a, b, d and e quadruple mutant (QK). QK is very sensitive to heat. Therefore, we used microarray to study how many genes regulated by HSFA1 after heat shock. Seven-day-old seedlings of Col-0, Ws and QK grown at 22oC on 0.5x MS plates containing 1% sucrose were incubated for 1 h at 37 °C. Subsequently, samples were collected for RNA extraction. The experiment was repeated, and two biological replicates were processed for analysis.
Experiment type
transcription profiling by array 
Contacts
Hsiang-chin Liu, Yee-yung Charng
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-26266.idf.txt
Sample and data relationshipE-GEOD-26266.sdrf.txt
Raw data (1)E-GEOD-26266.raw.1.zip
Processed data (1)E-GEOD-26266.processed.1.zip
Array designA-AFFY-2.adf.txt
R ExpressionSetE-GEOD-26266.eSet.r
Links