Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.
E-GEOD-26266 - Transcription profiling by array of Arabidopsis Col-0 and Ws ecotypes and QK mutants after heat-shock treatment
Released on 23 December 2010, last updated on 26 January 2012
In order to study the importance of HSFA1 in thermotolerance in Arabidopsis, we generated the HSFA1a, b, d and e quadruple mutant (QK). QK is very sensitive to heat. Therefore, we used microarray to study how many genes regulated by HSFA1 after heat shock. Seven-day-old seedlings of Col-0, Ws and QK grown at 22oC on 0.5x MS plates containing 1% sucrose were incubated for 1 h at 37 °C. Subsequently, samples were collected for RNA extraction. The experiment was repeated, and two biological replicates were processed for analysis.
transcription profiling by array
Hsiang-chin Liu, Yee-yung Charng
The role of class A1 heat shock factors (HSFA1s) in response to heat and other stresses in Arabidopsis. Liu HC, Liao HT, Charng YY. , PMID:21241330