5 protocols
AccessionType
normalization data transformation protocol
Raw CEL files were processed using Affymetrix Expression Console Software using RMA ID_REF = VALUE = log2 RMA signal
array scanning protocol
Standard Affymetrix WT protocol for Gene 1.0 ST arrays
hybridization protocol
Standard Affymetrix WT protocol for Gene 1.0 ST arrays
labelling protocol
Total RNA was biotin labeled using the NuGEN pico amplification protocol
nucleic acid extraction protocol
Glabrous hindpaw skin samples from euthanized transgenic animals were quickly dissesected free, placed on a small sponge to maintain a flattened orientation, and snap frozen in liquid N2 cooled isopentane. Samples were cryostat sectioned at 14mm thickness and placed onto a series of RNAse-free glass slides with suffiecient separation between adjacent section to allow for LCM. Slides were then quickly stained with Hematoxilin and Eosin, dehydrated through ethanols and xylene, and placed onto the AutoPixII LCM, where the epidermis of each sample was outlined. Once dry, the epidermis strips from each section were laser etched and captured for RNA extraction. Total RNA was extracted using the Arcturus Picopure isolation kit using manufacturer recommended protocols.