Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.

E-GEOD-26161 - Profiling of Argonaute (AGO) 2-associated sRNAs after infection of bacterial pathogen Pseudomonas syringae

Status
Released on 10 May 2011, last updated on 22 August 2013
Organism
Arabidopsis thaliana
Samples (4)
Protocols (3)
Description
Transgenic Arabidopsis plants (AGO2::HA:AGO2) were treated with either mock (10 mM MgCl2) or Pseudomonas syringae pv. tomato (Pst) expressing avrRpt2 (R2) at a concentration of 2 x 107 cfu/ml for 14 hours. sRNAs associated with AGO1 and AGO2 were co-immunoprecipitated using antibodies against either AGO1 (AGO1-IP), or HA (hemagglutinin) (AGO2-IP). As controls, we also gel-purified the 18-28 nt fraction of the total RNAs from an AGO2 mutant (ago2-1). The co-immunoprecipitated or gel-purified RNAs were cloned and sequenced by Illumina deep sequencing. Examination of AGO-associated sRNAs in pathogen-treated or control plants
Experiment type
other 
Contacts
Wei-Chi Wang <motloe@gmail.com>, Hailing Jin, Hongwei Zhao
Citation
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-26161.idf.txt
Sample and data relationshipE-GEOD-26161.sdrf.txt
Processed data (1)E-GEOD-26161.processed.1.zip
Additional data (1)E-GEOD-26161.additional.1.zip
Links