Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.
E-GEOD-26136 - Mammalian Dpy-30 regulates genomic H3K4 methylation and is essential for ES cell fate specification
Released on 1 February 2011, last updated on 2 June 2014
H3K4 methylation is associated with active genes and, along with H3K27 methylation, is part of a bivalent chromatin mark that typifies poised developmental genes in ESCs. However, its functional roles in ESC maintenance and differentiation are not established. Here we show that mammalian Dpy-30, a core subunit of SET1/MLL complexes, biochemically modulates H3K4 methylation in vitro, and directly regulates chromosomal H3K4me3 throughout the mammalian genome. Depletion of Dpy-30 does not affect ESC self-renewal, but significantly alters the differentiation potential of ESCs, particularly along the neural lineage. The differentiation defect is accompanied by defects in gene induction and H3K4 methylation at key developmental loci. Our results provide strong experimental evidence for the hypothesis that H3K4 methylation is an essential functional component of the bivalent mark during activation of developmental genes in ESCs. Total RNAs from control or knockdown cells before and after RA-mediated differentiation were subjected to Illumina microarray analyses. ChIP-enriched DNA from mouse ES cells was analyzed by Solexa sequencing.
ChIP-seq, transcription profiling by array
Hao Jiang <firstname.lastname@example.org>, H Jiang, R G Roeder
Role for Dpy-30 in ES cell-fate specification by regulation of H3K4 methylation within bivalent domains. Jiang H, Shukla A, Wang X, Chen WY, Bernstein BE, Roeder RG. , PMID:21335234