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E-GEOD-26114 - Combination of ABT-869 with SAHA on AML with FLT3-ITD

Status
Released on 31 May 2011, last updated on 27 March 2012
Organism
Homo sapiens
Samples (24)
Array (1)
Protocols (8)
Description
For the microarray experiments, MV4-11 and MOLM-14 cells were treated with DMSO control, ABT-869 3 nM, SAHA 6 M and combination therapy for 24 hours. Cells were then washed in PBS and high-quality total RNA was extracted RNeasy Midi Kit, according to the manufacturer’s instruction (Qiagen, Valencia, USA). RNA quantity, quality, and purity were assessed with the use of the RNA 6000 Nano assay on the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara CA, USA). Gene expression profiling was performed using Affymetric U133plus2.0 gene chip (Affymetrix, Santa Clara, CA, USA) according to the manufacturer’s protocol. MV4-11 and MOLM-14 cells were treated with DMSO control, ABT-869, SAHA or combination therapy for 24 hours.
Experiment type
transcription profiling by array 
Contacts
Jianbiao Zhou <jianbiaozhou@yahoo.com>, Chien-Shing Chen, Wee-Joo Chng
Citation
PRL-3, a Metastasis Associated Tyrosine Phosphatase, Is Involved in FLT3-ITD Signaling and Implicated in Anti-AML Therapy. Zhou J, Bi C, Chng WJ, Cheong LL, Liu SC, Mahara S, Tay KG, Zeng Q, Li J, Guo K, Tan CP, Yu H, Albert DH, Chen CS. , PMID:21589872
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-26114.idf.txt
Sample and data relationshipE-GEOD-26114.sdrf.txt
Raw data (1)E-GEOD-26114.raw.1.zip
Processed data (1)E-GEOD-26114.processed.1.zip
Array designA-AFFY-44.adf.txt
R ExpressionSetE-GEOD-26114.eSet.r
Links