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E-GEOD-25889 - Transcription profiling by array of Arabidopsis mutant for ced1

Status
Released on 8 December 2010, last updated on 26 January 2012
Organism
Arabidopsis thaliana
Samples (4)
Array (1)
Protocols (8)
Description
ced1 mutant has reduced expression of NCED3 in response to osmotic stress (polyethylene glycol) treatments compared to the wild type. Other ABA biosynthesis genes are also greatly reduced in ced1 under osmotic stress. We used microarrays to detail the global programme of gene expression under osmotic stress treatment and identified distinct classes of up/down-regulated genes in the stress pathway. Arabidopsis seedlings were selected at 16 days with 6hr PEG treatment for RNA extraction and hybridization on Affymetrix microarrays. From Norther data we have known some of well-known stress responsive gene were significantly changed. We sought to obtain whole gene expression to analysis which genes were affected in the osmotic stress pathway.
Experiment type
transcription profiling by array 
Contacts
zhenyu wang <wzy43155@gmail.com>, Jiankang Zhu, Zhenyu Wang
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-25889.idf.txt
Sample and data relationshipE-GEOD-25889.sdrf.txt
Raw data (1)E-GEOD-25889.raw.1.zip
Processed data (1)E-GEOD-25889.processed.1.zip
Array designA-AFFY-2.adf.txt
Links