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E-GEOD-25707 - Synergistic activation by p38MAPK and glucocorticoid signaling mediates induction of M2-like tumor-associated macrophages expressing the novel CD20 homolog MS4A8A

Status
Released on 3 January 2011, last updated on 27 March 2012
Organism
Mus musculus
Samples (9)
Array (1)
Protocols (8)
Description
Analysis of bone marrow derived macrophages (BMDM) incubated with dexamethasone&IL4 (Dexa+IL4), B16F1 tumor conditioned medium (cmB16), and B16F1 tumor conditioned medium supplemented with dexamethasone&IL4 (cmB16+dexa+IL4). Results allow detection of genes that require synergistic stimulation of tumor factors and Th2 cytokines. Bone marrow cells were isolated from the tibias and femurs of C57BL/6 mice. M-CSF at a concentration of 30 ng/ml was added to the culture medium [DMEM, 10% FCS, and penicillin/streptomycin]. After 4 days, the culture medium was replaced by fresh DMEM or B16F1 conditioned medium. As indicated, IL-4 (10 ng/ml), dexamethasone (5 × 10−7M), Iwas added. Cells were harvested 3 days later for RNA isolation.
Experiment type
transcription profiling by array 
Contacts
Carsten Sticht <geo@ncbi.nlm.nih.gov>, Alexandra Demory, Astrid Schmieder, Cleopatra Gkaniatsou, Cyrill Géraud, Jan P Nicolay, Jan P Tuckermann, Julia Kzhyshkowska, Julia Michel, Jörg Faulhaber, Kai Schledzewski, Lydia Tome, Sergij Goerdt
Citation
Synergistic activation by p38MAPK and glucocorticoid signaling mediates induction of M2-like tumor-associated macrophages expressing the novel CD20 homolog MS4A8A. Schmieder A, Schledzewski K, Michel J, Tuckermann JP, Tome L, Sticht C, Gkaniatsou C, Nicolay JP, Demory A, Faulhaber J, Kzhyshkowska J, Géraud C, Goerdt S. , PMID:20824698
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-25707.idf.txt
Sample and data relationshipE-GEOD-25707.sdrf.txt
Raw data (1)E-GEOD-25707.raw.1.zip
Processed data (1)E-GEOD-25707.processed.1.zip
Array designA-GEOD-6526.adf.txt
R ExpressionSetE-GEOD-25707.eSet.r
Links