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E-GEOD-25538 - EQUIVALENCE OF CONVENTIONALLY-DERIVED AND PARTHENOTE-DERIVED HUMAN EMBRYONIC STEM CELLS

Status
Released on 15 March 2011, last updated on 27 March 2012
Organism
Homo sapiens
Samples (39)
Array (1)
Protocols (6)
Description
Parthenote lines proliferated more slowly than conventional hESC lines, and yielded lower quantities of less mature differentiated cells in a neural progenitor cell (NPC) differentiation protocol. However, the cell lines performed similarly in a RPE differentiation protocol. The DNA methylation analysis showed similar general profiles, but the two cell types differed in methylation of imprinted genes. There were no major differences in gene expression between the lines before differentiation, but when differentiated into NPCs, the two cell types differed in expression of extracellular matrix (ECM) genes. Conclusions/Significance: These data show that hESC and phESC are similar in the undifferentiated state, and both cell types are capable of differentiation along neural lineages. The differences between the cell types, in proliferation and extent of differentiation, may be linked, in part, to the observed differences in ECM synthesis and methylation of imprinted genes. hESC and phESC were exposed to the same expansion conditions and subsequent neural and retinal pigmented epithelium (RPE) differentiation protocols. Growth rates and gross morphology were recorded during expansion. RTPCR for developmentally relevant genes and global DNA methylation profiling were used to compare gene expression and epigenetic characteristics.
Experiment type
methylation profiling by array 
Contacts
Julie V Harness <jharness@uci.edu>, David Ferguson, Gabriel Nistor, Gulsah Altun, Hans S Keirstead, Jeanne F Loring, Larisa S Agapova, Louise C Laurent, Magdalene J Seiler, Nikolay A Turovets
Citation
Equivalence of conventionally-derived and parthenote-derived human embryonic stem cells. Harness JV, Turovets NA, Seiler MJ, Nistor G, Altun G, Agapova LS, Ferguson D, Laurent LC, Loring JF, Keirstead HS. , PMID:21249129
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-25538.idf.txt
Sample and data relationshipE-GEOD-25538.sdrf.txt
Processed data (1)E-GEOD-25538.processed.1.zip
Array designA-GEOD-8490.adf.txt
Links