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E-GEOD-25334 - Asymmetric self-renewal associated (ASRA) genes

Status
Released on 22 December 2010, last updated on 26 June 2012
Organism
Mus musculus
Samples (8)
Array (1)
Protocols (8)
Description
Cell lines geneticially engineered to undergo conditional asymmetric self-renewal were used to identify genes whose expression is asymmetric self-renewal associated (ASRA). Non-random sister chromatid segregation occurs concordantly with asymmetric self-renewal in these cell lines. Asymmetric self-renewal occurs when murine embryo fibroblasts that are otherwise p53-null are induced to express physiological levels of wildtype p53 protein (Asym). To distinguish p53-responsive genes that also require induction of asymmetric self renewal (i.e., ASRA genes) and/or non-random sister chromatid segregation for change, an additional control cell line, which continues to symmetrically self-renew (with random sister chromatid segregation) even when p53 is induced, was also compared (Symp53). This congenic cell line constitutively expresses the type II inosine monophosphate dehydrogenase (IMPDH II; the rate-limiting enzmye for guanine ribonucleotide biosynthesis) and, thereby, prevents p53-induced asymmetric self-renewal and non-random sister chromatid segregation. Three biological replicates of asymmetrically self-renewing cultures (Asym1-3) were compared with cultures that were symmetrically self-renewing - either because they did not express p53 (3 biological replicates, Sym1-3) or they expressed constitutive IMPDH II (i.e., not regulated by p53) as well as p53 (2 biological replicates, Symp53_1 and 2.)
Experiment type
transcription profiling by array 
Contacts
James Sherley <janet.l.smith@gmail.com>, James L Sherley, Janet L Smith, Minsoo Noh
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-25334.idf.txt
Sample and data relationshipE-GEOD-25334.sdrf.txt
Raw data (1)E-GEOD-25334.raw.1.zip
Processed data (1)E-GEOD-25334.processed.1.zip
Array designA-GEOD-10773.adf.txt
R ExpressionSetE-GEOD-25334.eSet.r
Links