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E-GEOD-25148 - Changes in gene expression in HEK-TLR2 cells in response to Helicobacter pylori lipopolysaccharide

Status
Released on 12 January 2011
Organism
Homo sapiens
Samples (20)
Array (1)
Protocols (8)
Description
This study set out to identify global changes in gene expression in human embryonic kidney (HEK) cells stably transfected with Toll-like receptor 2 (TLR2) over a 48 hour time-course, following stimulation with 10 μg/ml lipopolysaccharide (LPS) from the gastric pathogen H. pylori. Microarray analysis was used to compare changes in gene expression between cells treated with 10 μg/ml H. pylori LPS and untreated cells at the same time point. Total RNA was harvested from HEK-TLR2 cells following treatment with LPS for 2, 4, 8, 24 and 48h or untreated cells at the same time-point. 2 independent experiments were carried out. RNA was labeled and hybridized to GeneChips to analyse changes in gene expression.
Experiment type
transcription profiling by array 
Contacts
Sinead Marian Smith <geo@ncbi.nlm.nih.gov>, Anthony P Moran, Dermot P Kelleher, Shane P Duggan, Sinead M Smith
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-25148.idf.txt
Sample and data relationshipE-GEOD-25148.sdrf.txt
Raw data (1)E-GEOD-25148.raw.1.zip
Processed data (1)E-GEOD-25148.processed.1.zip
Array designA-AFFY-44.adf.txt
R ExpressionSetE-GEOD-25148.eSet.r
Links