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E-GEOD-2509 - Transcription profiling of two human colon cancer cell lines derived from a primry tumor and a lypmh node metastatsis from a single patient reveals global alterations in mRNA polysomal recruitment in a cell model of colorectal cancer progression to metastasis

Status
Released on 27 October 2007, last updated on 27 March 2012
Organism
Homo sapiens
Samples (6)
Array (1)
Protocols (2)
Description
Two colon cancer cell lines are under study. SW480 and SW620. The first one is derived from primary cancer, SW620 are from lymphnode metastatic sites. they both come from the sample patient. Polysomal RNA fractions from the two isogenic colon cancer cells lines was purified by sucrose gradient and hybridized on affymetrix hgu133a chips. this study is complementary to the series GSE1323 where total RNA was used instead. Comparison between the polysomal fraction chips and the total RNA chips is performed and the analysis proposed in a paper from the authors (at the moment in preparation).
Experiment types
transcription profiling by array, co-expression, disease state, in vitro
Contact
Citation
Global alterations in mRNA polysomal recruitment in a cell model of colorectal cancer progression to metastasis. Alessandro Provenzani, Raffaele Fronza, Fabrizio Loreni, Alessia Pascale, Marialaura Amadio, Alessandro Quattrone. Carcinogenesis 27(7):1323-33 (2006)
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-2509.idf.txt
Sample and data relationshipE-GEOD-2509.sdrf.txt
Raw data (1)E-GEOD-2509.raw.1.zip
Processed data (1)E-GEOD-2509.processed.1.zip
Array designA-AFFY-33.adf.txt
R ExpressionSetE-GEOD-2509.eSet.r
Links