Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.
E-GEOD-25011 - Study for evaluating the effect of cold ischemic time and RNA stabilization method on RNA integrity and gene expression measurements
Released on 2 November 2011, last updated on 5 December 2011
Time series of eleven breast cancer samples subjected to different cold ischemic stress of up to 3 hr post tumor excision. A different 2x2 factorial within this study evaluated the effect of stabilization method (RNAlater vs snap freezing) and stablization delay (0 and 40 min) at room temperature. Tissue samples were collected at surgery, cut into 1-2 mm pieces and divided into 8 portions. Portions were put in RNAlater or fresh frozen at baseline, 20, 40, 60, 120, and 180 minutes thereafter, or snap frozen in dry ice in a pre-chilled sample vial at baseline and 40 minutes thereafter.
transcription profiling by array
Christos Hatzis, Hongxia Sun, W F Symmans
Effects of Tissue Handling on RNA Integrity and Microarray Measurements From Resected Breast Cancers. Hatzis C, Sun H, Yao H, Hubbard RE, Meric-Bernstam F, Babiera GV, Wu Y, Pusztai L, Symmans WF. , PMID:22034635