VALUE = normalized signal
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
293T cells were grown on 10cm plates in DMEM containing 10% FBS under standard TC conditions
The data were normalized and analyzed with Genepattern software.
Total RNA was extracted 48 hrs post transfection by Trizol extraction according to the manufacturer's instructions.
GeneChips were scanned using GeneArray Scanner.
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
293T cells were transfected either by pCMV-Aire (6ug/plate) or empty pCMV (6ug/plate) using Mirus reagent, according to manufacturer's instructions