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E-GEOD-24733 - Gene Expression Profile of 293T and 293T-Aire-expressing cells

Status
Released on 16 October 2010, last updated on 10 June 2011
Organism
Homo sapiens
Samples (5)
Array (1)
Protocols (8)
Description
To analyze the impact of Aire on gene expression profile in a model cell line, we used 293T cells and transfected them either with an Aire expression plasmid pCMV-Aire (where mAire is driven by CMV promoter) or with a control plasmid pCMV2B. Total RNA was extracted 48 hours post transfection, processed and used for gene expression profiling by Affymetrix. The data demonstrate that Aire has a very broad impact, effecting (upregulating and downregulating) hundreds of differents genes, however these genes differ dramatically from its targets in medullary epithelial cells. Keywords: transfection 293T cells were seeded on 10cm plates and were grown in DMEM containing 10% FBS under standard TC conditions. Next day, the cells were transfected either by pCMV-Aire (6ug/plate) or empty pCMV (6ug/plate) using Mirus reagent, according to manufacturer's instructions. Total RNA was extracted 48 hrs post transfection by Trizol protocol according to the manufacturer's instructions. 3 biological replicates were used for Aire transfection and 2 replicates for transfection with a control plasmid.
Experiment type
transcription profiling by array 
Contacts
CBDM Z. Lab <cbdm@joslin.harvard.edu>, Christophe Benoist, Diane Mathis, Jakub Abramson
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-24733.idf.txt
Sample and data relationshipE-GEOD-24733.sdrf.txt
Raw data (1)E-GEOD-24733.raw.1.zip
Processed data (1)E-GEOD-24733.processed.1.zip
Array designA-AFFY-44.adf.txt
R ExpressionSetE-GEOD-24733.eSet.r
Links