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E-GEOD-24633 - Cdx2 transcription factor binding in intestinal villus and gene expression profiling in Cdx mutant mice

Status
Released on 13 April 2011, last updated on 2 June 2014
Organism
Mus musculus
Samples (13)
Array (1)
Protocols (11)
Description
We conditionally inactivated mouse Cdx2, a dominant regulator of intestinal development, and mapped its genome occupancy in adult intestinal villi. Although homeotic transformation, observed in Cdx2-null embryos, was absent in mutant adults, gene expression and cell morphology were vitally compromised. Lethality was accelerated in mice lacking both Cdx2 and its homolog Cdx1, with exaggeration of defects in crypt cell replication and enterocyte differentiation. Cdx2 occupancy correlated with hundreds of transcripts that fell but not with equal numbers that rose with Cdx loss, indicating a predominantly activating role at intestinal cis-regulatory regions. Integrated consideration of a mutant phenotype and cistrome hence reveals the continued and distinct requirement in adults of a master developmental regulator that activates tissue-specific genes. Cdx2 ChIP-seq in mouse villus, and gene expression data from Cdx1, Cdx2 and compound knockout mouse intestine
Experiment types
ChIP-seq, transcription profiling by array 
Contacts
Michael Verzi <geo@ncbi.nlm.nih.gov>, Hyunjin Shin, Michael P Verzi, Ramesh A Shivdasani, X S Liu
Citation
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
MIAME
PlatformsProtocolsVariablesProcessedRaw
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