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E-GEOD-24471 - ChIP-Seq data in secondary fibroblast with dox-inducible cassettes for Oct4, Sox2 and Klf4

Status
Released on 1 October 2010, last updated on 3 April 2013
Organism
Mus musculus
Samples (5)
Protocols (3)
Description
The conversion of fibroblasts to induced pluripotent stem cells (iPS) by forced expression of Oct4, Sox2 and Klf4 is among the earliest demonstrations of reprogramming to a pluripotent state by forced expression of transcription factors. To gain insights into the transcriptional state of genes required for reprogramming, we profiled RNA polymerase II, H3K27me3 and E2F4. DNA was enriched by chromatin immunoprecipitation (ChIP) and analyzed by Solexa sequencing. ChIP was performed using an antibodies to RNA polymerase II, H3K27me3 and E2F4.
Experiment type
ChIP-seq 
Contacts
Richard A Young, Steve Bilodeau
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-24471.idf.txt
Sample and data relationshipE-GEOD-24471.sdrf.txt
Processed data (1)E-GEOD-24471.processed.1.zip
Links