E-GEOD-24422 - Transcription profiling by array of human mesenchymal stem cell derived stromal adipocytes after treatment with insulin and/or tumor necrosis factor

Status
Released on 30 November 2010, last updated on 3 April 2013
Organism
Homo sapiens
Samples (24)
Array (1)
Protocols (8)
Description
There are an estimated 21million diabetics in the United States and 150 million diabetics worldwide. The World Health Organization anticipates that these numbers will double in the next 20 years. Metabolic syndrome is a well recognized set of symptoms that increases a patient’s risk of developing diabetes. Insulin resistance is a factor in both metabolic syndrome and Type 2 diabetes. It is characterized by decreased insulin stimulated glucose uptake in peripheral tissues, decreased adiponectin levels, increased adipocyte FFA and cytokine production, and increased insulin and hepatic glucose output. Prevention or reversal of insulin resistance should serve as an important strategy in addressing the growing health concerns posed by the Diabetes epidemic. While increased adiposity is associated with insulin resistance, the role of the cell types present within adipose (adipocytes, pre-adipocytes, endothelial cells, macrophages, fibroblasts, leukocytes and smooth muscle cells) in insulin resistance is unclear. In an effort to begin dissection of this question, we examined the transcriptional response of the buoyant and non-buoyant fractions isolated from insulin sensitive or TNF induced insulin resistant hMSC derived adipocytes before and after treatment with insulin. hMSC derived adipocytes were treated with 0 or 10ng/ml TNF for 24 hours to induce insulin resistance and subsequently serum starved for 5 h followed by treatment with 0 or 20nM insulin for 2 hours. At the end of the incubation period, cells were harvested as is (mixed) or subjected to fractionation to separate the adipocytes (buoyant fraction) and the stromal cells (non-buoyant fraction). These isolated cells were resuspended in RLT buffer to prepare lysates for total RNA isolation using the Qiagen RNeasy kit. Total RNA was submitted to GeneLogic for cRNA preparation using the Affymetrix GenChip IVT kit and hybridization to Affymetrix U133 Plus 2.0 arrays.
Experiment type
transcription profiling by array 
Contacts
Shelley des Etages <shelley.g.desetages@pfizer.com>, Amy Baumann, Andrew Kuhn, Lisa S Hayes, Mark A Thiede, Mary K Bauchmann, Shelley G desEtages
Citation
Genome-wide profiling of H3K56 acetylation and transcription factor binding sites in human adipocytes. Lo KA, Bauchmann MK, Baumann AP, Donahue CJ, Thiede MA, Hayes LS, des Etages SA, Fraenkel E. , Europe PMC 21655096
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-24422.idf.txt
Sample and data relationshipE-GEOD-24422.sdrf.txt
Raw data (1)E-GEOD-24422.raw.1.zip
Processed data (1)E-GEOD-24422.processed.1.zip
Array designA-AFFY-44.adf.txt
Links