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E-GEOD-24327 - Lack of Toll-like Receptor Signaling Improves Host Defense in Severe Septic Peritonitis in Severe Septic Peritonitis

Status
Released on 27 June 2011, last updated on 26 June 2012
Organism
Mus musculus
Samples (12)
Array (1)
Protocols (6)
Description
TLRs are considered important for innate immune responses that combat bacterial infections. Here, the role of TLRs in severe septic peritonitis using the colon ascendens stent peritonitis (CASP) model was examined. We demonstrate that mice deficient for MyD88 and TRIF had markedly reduced bacterial numbers both in peritoneal cavity and peripheral blood, indicating that bacterial clearance in this model is inhibited by TLR signals. Moreover, survival of Myd88-/-;TrifLps2/Lps2 mice was significantly improved. The lack of TLR signals prevented the excessive induction of inflammatory cytokines and of IL 10. Notably, the expression of IFN-gamma, which has an essential protective role in septic peritonitis, and of IFN-regulated genes including several p47 and p65 GTPases as well as IP 10 was independent of TLR signaling. These results provide evidence that, in severe septic peritonitis, TLR deficiency balances the innate immune response in a favorable manner by attenuating deleterious responses such as excessive cytokine release, while leaving intact protective IFN-gamma production. In this dataset, expression data of genes induced by septic peritonitis in spleens from TLR-deficient and wildtype mice are included. 3 groups (septic TLR-deficient mice, septic wildtype mice, and untreated wildtype mice) with 4 replicates each.
Experiment type
transcription profiling by array 
Contacts
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-24327.idf.txt
Sample and data relationshipE-GEOD-24327.sdrf.txt
Raw data (1)E-GEOD-24327.raw.1.zip
Processed data (1)E-GEOD-24327.processed.1.zip
Array designA-AFFY-130.adf.txt
R ExpressionSetE-GEOD-24327.eSet.r
Links